ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : A Preliminary Study on Expression of Zinc Transporter Gene of Cassava Grown in Nutrient Solutions with some Physiological and Biochemical Responses)

ผู้เขียน:Phanuphong Khongchiu, ดร.วิจารณ์ วิชชุกิจ, รองศาสตราจารย์, Pasajee Kongsila, ดร.ประศาสตร์ เกื้อมณี, รองศาสตราจารย์, Arunee Wongkeaw, ดร.สุตเขตต์ นาคะเสถียร, รองศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

Cassava growth can be affected by either zinc (Zn) defi ciency or excess supply throughout the crop cycle. In this study, cassava plants (cv. KU50) were grown in Enshi’s nutrient solutions combined with three Zn levels (0, 1.0 and 10.0 ?M) in a plastic house. Procedures covering sampling and replacement with fresh nutrient solutions were conducted weekly. The results indicated that the Zn application at 10.0 ?M produced a signifi cant increase in shoot and total dry matter. The dry weight shoot-root ratio showed a signifi cant increase at all Zn levels. Thus, Zn nutrient promoted shoots more than roots during the early growth stage of cassava. An increased Zn supply increased the cassava Zn accumulation and Zn uptake, but decreased the Zn utilization effi ciency. Under different Zn conditions, the partial MeZIP gene was observed in all plant parts. At a Zn-defi cient level, the transcription patterns of the partial MeZIP showed high expression in the roots rather than in the stems and leaves. The differential expression of the MeZIP gene corresponded to the different Zn levels. This implied that the partial MeZIP gene refl ected plant performance to overcome the Zn defi ciency.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 048, Issue 4, Jul 14 - Aug 14, Page 515 - 524 |  PDF |  Page

ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Cloning and Molecular Characterization of the Zinc Transporter (ZIP) Gene from Cassava)

ผู้เขียน:ธนวรรธห์ บำรุงเศรษฐพงษ์, ดร.วิจารณ์ วิชชุกิจ, รองศาสตราจารย์, ดร.สุตเขตต์ นาคะเสถียร, รองศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

The zinc transporter (ZIP) involved in zinc uptake, controls the major steps in growth development tissue. In this study, cassava (Manihot esculenta Crantz var. HB80) ZIP cDNA of 726 bp length was isolated from developing fibrous roots during growth in tissue culture. This gene was highly homologous with those from other plant species. DNA blot analysis indicated that at least two copies of ZIP are present in the cassava genome. Three Thai cassava varieties, namely Huaybong 80 (HB80), Kasetsart 50 (KU50) and Rayong 1 (R1) were used to evaluate the steady-state transcript accumulation of transporter genes in a tissue culture experiment. Their agronomic characters were also observed. Total dry weight (TDW) of each variety was found to be highest after eight weeks culture with 0.15 mM ZnSO4.7H2O added to the culture medium. The lengths of leaves, petioles, stems and fibrous roots were all found to be greatest with this treatment level. A high differential expression of the ZIP gene in different tissue media was observed in the stems, fibrous roots and leafs, respectively. The results showed that the levels of ZIP genes were high in the treatment containing 0 mM and 0.15 mM of ZnSO4.7H2O, which was reflected by the highest fresh mass, dry mass, leaf number, fibrous root number, leaf length, stem length, petiole length and fibrous root length with the 0.15 mM ZnSO4.7H2O treatment. The high level expression of these genes was most pronounced five to six weeks after harvest, because the plants used more nutrients at the start of growth development. The levels of these genes in R1 showed a higher level of expression than those of KU50 and HB80, reflected by R1 having the highest fresh mass when compared with KU50 and HB80.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 044, Issue 5, Sep 10 - Oct 10, Page 891 - 901 |  PDF |  Page